Automated cell lineage tracing in Caenorhabditis elegans
نویسندگان
چکیده
منابع مشابه
Automated cell lineage tracing in Caenorhabditis elegans.
The invariant cell lineage and cell fate of Caenorhabditis elegans provide a unique opportunity to decode the molecular mechanisms of animal development. To exploit this opportunity, we have developed a system for automated cell lineage tracing during C. elegans embryogenesis, based on 3D, time-lapse imaging and automated image analysis. Using ubiquitously expressed histone-GFP fusion protein t...
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Describing gene expression during animal development requires a way to quantitatively measure expression levels with cellular resolution and to describe how expression changes with time. Fluorescent protein reporters make it possible to measure expression dynamics in live cells by time-lapse microscopy, but it can be challenging to identify expressing cells in complex tissues and to compare exp...
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“The relationship between genes and development is unknown.” This is the bold opening statement of the 1980 article by H. Robert Horvitz and John Sulston that reported the first step of a comprehensive genetic analysis of the Caenorhabditis elegans cell lineage. In this article, Horvitz and Sulston demonstrated that systematic mutational analysis could dissect the regulation of the precise cell...
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Advances in microscopy and fluorescent reporters have allowed us to detect the onset of gene expression on a cell-by-cell basis in a systemic fashion. This information, however, is often encoded in large repositories of images, and developing ways to extract this spatiotemporal expression data is a difficult problem that often uses complex domain-specific methods for each individual data set. W...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2006
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0511111103